Journal: Fish and Shellfish Immunology Reports
Article Title: Nutritional feed additives reduce the adverse effects of transport stress in the immune system of Tambaqui (Colossoma macropomum)
doi: 10.1016/j.fsirep.2022.100051
Figure Lengend Snippet: Hemoglobin concentration (a), red blood cell (RBC) count (b), hematocrit (c), total leukocyte count (TLC) (d) of juvenile tambaqui fed the experimental diets before and after transport stress and at 30 days post-transport. Capital letters on the top of bars compare the treatments in each time point, while small letters inside the bars evaluate the effect of the sampling points in each dietary treatment using Bonferroni's multiple range test ( p <0.05). Values are means of two determinations per fish, two fish per tank and four tanks per treatment. Space between the dotted lines indicates the reference interval for healthy tambaqui (Ranzani-Paiva et al. 1998, 1999; Tavares-Dias & Sandrin 1998; Tavares-Dias et al. 1998).
Article Snippet: Hematocrit was determined by centrifuging a blood sample at 8000 rpm for 5 min. Serum protein was determined using a colourimetric commercial kit (Labtest Diagnostica®) based on the biuret method.
Techniques: Concentration Assay, Sampling